check point 1 check point 2 check point 3 check point 4 check point 5 check point 6 check point 7 check point 8 check point 9 check point 10 check point 11 check point 12 25C 30C 37C nutrient R2A NH4/NO2 oxidizers H2S,So oxidizers Fe oxidizers(acidic pH) Fe oxidizers(neutral pH) Mn oxidizers Methanogenic enrichment SRB R2A+nitrate nutrient+nitrate rich sugar total #'s colony morphotypes gram stain check point 1 check point 2 check point 3 check point 4 check point 5 check point 6 check point 7 check point 8 check point 9 check point 10 check point 11 check point 12 all bacteria denitrifiers methanogens methanotrophs nitrifiers Fe oxidizers sulfur oxidizers sulfate reducers check point 1 check point 2 check point 3 check point 4 check point 5 check point 6 check point 7 check point 8 check point 9 check point 10 check point 11 check point 12 all bacteria methanogens nitrifiers Fe oxidizers sulfate reducers check point 1 check point 2 check point 3 check point 4 check point 5 check point 6 check point 7 check point 8 check point 9 check point 10 check point 11 check point 12 check point 1 check point 2 check point 3 check point 4 check point 5 check point 6 check point 7 check point 8 check point 9 check point 10 check point 11 check point 12 pH dissolved oxygen PO4 Cl- H2S SO4 NO3 NO2 NH4